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Ses suppresses APAP-induced ferroptosis in mice. (a,b) Flow cytometric analysis and quantification of mitochondrial membrane potential (MMP) in mouse kidney. (c) Iron content was detected in kidney tissues. (d–k) Western blot bands showing the protein expressions and relative signal intensities of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), transferrin receptor protein 1 (TfR1), <t>ferroportin1</t> <t>(FPN1),</t> ferritin light chain (FTL), and ferritin heavy chain 1 (FTH1) in kidney tissue. For quantification, the intensity was normalized to β -actin and the control was set to 1. Values are expressed as the mean ± SEM ( n = 6); * p < 0.05 difference from the control group; ** p < 0.01 difference from the control group; *** p < 0.001 difference from the control group; # p < 0.05 difference from the APAP exposure group; ## p < 0.01 difference from the APAP exposure group; and ### p < 0.001 difference from the APAP exposure group.
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Ses suppresses APAP-induced ferroptosis in mice. (a,b) Flow cytometric analysis and quantification of mitochondrial membrane potential (MMP) in mouse kidney. (c) Iron content was detected in kidney tissues. (d–k) Western blot bands showing the protein expressions and relative signal intensities of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), transferrin receptor protein 1 (TfR1), <t>ferroportin1</t> <t>(FPN1),</t> ferritin light chain (FTL), and ferritin heavy chain 1 (FTH1) in kidney tissue. For quantification, the intensity was normalized to β -actin and the control was set to 1. Values are expressed as the mean ± SEM ( n = 6); * p < 0.05 difference from the control group; ** p < 0.01 difference from the control group; *** p < 0.001 difference from the control group; # p < 0.05 difference from the APAP exposure group; ## p < 0.01 difference from the APAP exposure group; and ### p < 0.001 difference from the APAP exposure group.
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Ses suppresses APAP-induced ferroptosis in mice. (a,b) Flow cytometric analysis and quantification of mitochondrial membrane potential (MMP) in mouse kidney. (c) Iron content was detected in kidney tissues. (d–k) Western blot bands showing the protein expressions and relative signal intensities of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), transferrin receptor protein 1 (TfR1), <t>ferroportin1</t> <t>(FPN1),</t> ferritin light chain (FTL), and ferritin heavy chain 1 (FTH1) in kidney tissue. For quantification, the intensity was normalized to β -actin and the control was set to 1. Values are expressed as the mean ± SEM ( n = 6); * p < 0.05 difference from the control group; ** p < 0.01 difference from the control group; *** p < 0.001 difference from the control group; # p < 0.05 difference from the APAP exposure group; ## p < 0.01 difference from the APAP exposure group; and ### p < 0.001 difference from the APAP exposure group.
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Ses suppresses APAP-induced ferroptosis in mice. (a,b) Flow cytometric analysis and quantification of mitochondrial membrane potential (MMP) in mouse kidney. (c) Iron content was detected in kidney tissues. (d–k) Western blot bands showing the protein expressions and relative signal intensities of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), transferrin receptor protein 1 (TfR1), <t>ferroportin1</t> <t>(FPN1),</t> ferritin light chain (FTL), and ferritin heavy chain 1 (FTH1) in kidney tissue. For quantification, the intensity was normalized to β -actin and the control was set to 1. Values are expressed as the mean ± SEM ( n = 6); * p < 0.05 difference from the control group; ** p < 0.01 difference from the control group; *** p < 0.001 difference from the control group; # p < 0.05 difference from the APAP exposure group; ## p < 0.01 difference from the APAP exposure group; and ### p < 0.001 difference from the APAP exposure group.
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Ses suppresses APAP-induced ferroptosis in mice. (a,b) Flow cytometric analysis and quantification of mitochondrial membrane potential (MMP) in mouse kidney. (c) Iron content was detected in kidney tissues. (d–k) Western blot bands showing the protein expressions and relative signal intensities of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), transferrin receptor protein 1 (TfR1), ferroportin1 (FPN1), ferritin light chain (FTL), and ferritin heavy chain 1 (FTH1) in kidney tissue. For quantification, the intensity was normalized to β -actin and the control was set to 1. Values are expressed as the mean ± SEM ( n = 6); * p < 0.05 difference from the control group; ** p < 0.01 difference from the control group; *** p < 0.001 difference from the control group; # p < 0.05 difference from the APAP exposure group; ## p < 0.01 difference from the APAP exposure group; and ### p < 0.001 difference from the APAP exposure group.

Journal: Redox Report : Communications in Free Radical Research

Article Title: Sesamin protects against Acetaminophen-induced nephrotoxicity by suppressing HMOX1-mediated apoptosis and ferroptosis

doi: 10.1080/13510002.2025.2529695

Figure Lengend Snippet: Ses suppresses APAP-induced ferroptosis in mice. (a,b) Flow cytometric analysis and quantification of mitochondrial membrane potential (MMP) in mouse kidney. (c) Iron content was detected in kidney tissues. (d–k) Western blot bands showing the protein expressions and relative signal intensities of solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), transferrin receptor protein 1 (TfR1), ferroportin1 (FPN1), ferritin light chain (FTL), and ferritin heavy chain 1 (FTH1) in kidney tissue. For quantification, the intensity was normalized to β -actin and the control was set to 1. Values are expressed as the mean ± SEM ( n = 6); * p < 0.05 difference from the control group; ** p < 0.01 difference from the control group; *** p < 0.001 difference from the control group; # p < 0.05 difference from the APAP exposure group; ## p < 0.01 difference from the APAP exposure group; and ### p < 0.001 difference from the APAP exposure group.

Article Snippet: The primary antibodies against β -actin (AC026, 1:50000), solute carrier family 7 member 11 (SLC7A11, A2413, 1:2000), glutathione peroxidase 4 (GPX4, A1933, 1:1500), transferrin receptor protein 1 (TfR1, A5865, 1:1500), ferroportin1 (FPN1, A14884), ferritin light chain (FTL, A11241, 1:1500), ferritin heavy chain 1 (FTH1, A19544, 1:1500) and HMOX1 (A19062, 1:2000) were all sourced from ABclonal (Wuhan, China).

Techniques: Membrane, Western Blot, Control